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eLibrary > BS ISO 20631:2024 Infant formula and adult nutritionals. Determination of total folate content by trienzyme extraction and ultra high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) >

BS ISO 20631:2024 Infant formula and adult nutritionals. Determination of total folate content by trienzyme extraction and ultra high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS), 2024

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BS ISO 20631:2024 Infant formula and adult nutritionals. Determination of total folate content by trienzyme extraction and ultra high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS), 2024

undefined
Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents and materials [Go to Page]
5.1 List of reagents
5.2 Preparation of solvent for standard solutions
5.3 Preparation of folate standard solutions
5.4 Preparation of folate internal standard stock solutions
5.5 Preparation of calibration standard solutions
5.6 Preparation of substrate solution to test plasma conjugase activity
5.7 Reagent for folate analysis
6 Apparatus
7 Procedure [Go to Page]
7.1 Sample preparation [Go to Page]
7.1.1 Sample processing to make them homogenous
7.1.2 Reconstitution of powder sample into liquid
7.2 Extraction
7.3 Extract purification
7.4 Instrumental analysis [Go to Page]
7.4.1 Analysis of instrument blank, calibration standards, method blank and samples
7.4.2 UHPLC conditions and MS parameters for different systems
8 Calculations [Go to Page]
8.1 Calculation of ratios of peak areas of the folate compounds to the respective internal standard peak areas
8.2 Calibration curve
8.3 Calculation of concentration of folate compounds in the method blank
8.4 Calculation of concentration of folate compounds in the sample extracts
8.5 Calculation of folate compounds and total folate in reconstituted or RTF samples (μg/100 g)
8.6 Calculation of total folate in powder samples (μg/100 g) as-is basis
8.7 Calculation of folic acid nanograms released in the conjugase test
8.8 Calculation of per cent conversion of Pte-Glu3 to folic acid in the conjugase assay
9 Precision [Go to Page]
9.1 General
9.2 Repeatability
9.3 Reproducibility
Annex A (informative) Precision data
Bibliography [Go to Page]

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BS ISO 20631:2024 Infant formula and adult nutritionals. Determination of total folate content by trienzyme extraction and ultra high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS), 2024

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