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eLibrary > BS ISO 23040:2021 Marine environment impact assessment (MEIA). Specification for marine sediments in seabed areas. Survey of interstitial biota >

BS ISO 23040:2021 Marine environment impact assessment (MEIA). Specification for marine sediments in seabed areas. Survey of interstitial biota, 2022

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BS ISO 23040:2021 Marine environment impact assessment (MEIA). Specification for marine sediments in seabed areas. Survey of interstitial biota, 2022

National foreword
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 General [Go to Page]
4.1 Technical design
4.2 Basic recommendations for the surveys [Go to Page]
4.2.1 Survey object
4.2.2 Auxiliary parameters
4.2.3 Recommendations for the sampling equipment
4.2.4 Auxiliary equipment on board ship
4.2.5 Sampling method and scope of application
4.3 Sampling [Go to Page]
4.3.1 Sediment sampling
4.3.2 Trawl sampling
4.3.3 Water sampling
4.3.4 Records
4.4 Sample analysis [Go to Page]
4.4.1 Sample treatment
4.4.2 Sample measurement
4.4.3 Sample treatment and storage
4.4.4 Sample identification and enumeration
4.4.5 Sample data analysis
4.5 Basic recommendations of data organization [Go to Page]
4.5.1 Organization of data
4.5.2 Data archiving, acceptance and achievements appraisal
4.6 Survey results [Go to Page]
4.6.1 Original records
4.6.2 Maps or drawings
4.6.3 Investigation report
4.7 Data archiving
4.8 Program and quality control
5 Survey of the sediment [Go to Page]
5.1 Principle
5.2 General provisions
5.3 Collection and preservation of the samples
5.4 Measurement of environmental factors
5.5 Measurement of the age of the sediments
5.6 Measurement of the contents of heavy metals, organic pollutants and oils in the sediments
5.7 Measurement of the grain size of the sediments
5.8 Analysis of the mineral compositions in the sediments
5.9 Classification of the substrate type in offshore sediments
5.10 Classification of the deep-sea sediments
5.11 Survey of the biological components in the sediments [Go to Page]
5.11.1 Summary of the methods
5.11.2 Technical recommendations
5.11.3 Collection and preservation of the samples
5.12 Organization of data
6 Survey of foraminifera [Go to Page]
6.1 Principle
6.2 General provisions
6.3 Collection and preservation of the samples [Go to Page]
6.3.1 Sampling and sample processing
6.3.2 Collection and preservation of the sedimentary tests
6.3.3 Collection and preservation of living foraminifera
6.4 Tools and reagents
6.5 Processing and analysis of the samples [Go to Page]
6.5.1 Numbering and weighing the crystallizing dish or beaker
6.5.2 Drying and weighing the sediment sample
6.5.3 Sample soaking
6.5.4 Washing and drying
6.5.5 Suspension and concentration
6.5.6 Bottling and sealing
6.5.7 Specimen preparation and analysis
7 Survey of ostracoda [Go to Page]
7.1 Principle
7.2 General provisions
7.3 Collection and preservation of the samples [Go to Page]
7.3.1 Sampling and sample processing
7.3.2 Collection and preservation of dead ostracod tests
7.3.3 Collection and preservation of living ostracods
7.4 Tools and reagents
7.5 Processing and analysis of the samples [Go to Page]
7.5.1 Numbering and weighing the crystallizing dish or beaker
7.5.2 Drying and weighing
7.5.3 Sample soaking
7.5.4 Washing and drying
7.5.5 Suspension and concentration
7.5.6 Bottling and sealing
7.5.7 Microscopic examination and enumeration
8 Survey of radiolaria [Go to Page]
8.1 Principle
8.2 General provisions
8.3 Collection and preservation of the samples [Go to Page]
8.3.1 Sample processing
8.3.2 Collection and conservation of deposited empty shells
8.4 Tools and reagents
8.5 Processing and analysis of the samples [Go to Page]
8.5.1 Sample pretreatment
8.5.2 Preparation of microscope slide specimens
9 Survey of sedimentary diatom [Go to Page]
9.1 Principle
9.2 General provisions
9.3 Collection and preservation of the samples [Go to Page]
9.3.1 Sampling and treatment
9.3.2 Collection and preservation of sedimentary remains
9.4 Tools and reagents
9.5 Processing and analysis of the samples [Go to Page]
9.5.1 Sample pretreatment
9.5.2 Preparation of microscope slide specimens
9.6 Organization of data
10 Survey of coccoliths [Go to Page]
10.1 Principle
10.2 General provisions
10.3 Collection and preservation of the samples
10.4 Tools and reagents
10.5 Processing and analysis of the samples [Go to Page]
10.5.1 Pre-preparation of the equipment and the samples
10.5.2 Method of preparing simple smear slides
10.5.3 Pre-concentration of coccolith samples
10.6 Organization of data
11 Survey of sporopollen [Go to Page]
11.1 Principle
11.2 General provisions
11.3 Collection and preservation of the samples
11.4 Tools and reagents
11.5 Processing and analysis of the samples [Go to Page]
11.5.1 Sample disaggregation
11.5.2 Alkali-soluble digestion
11.5.3 Sieving
11.5.4 Carbonate digestion
11.5.5 Silicate digestion
11.5.6 Ultrasonic cleaning and sieving
11.5.7 Acetolysis
11.5.8 Storage of processed sample
11.5.9 Mounting sporopollen specimens
11.5.10 Glycerine jelly preparation
11.5.11 Precautions
11.6 Organization of data
12 Survey of benthic viruses [Go to Page]
12.1 Principle
12.2 General provisions
12.3 Collection and preservation of the samples [Go to Page]
12.3.1 Samples for epifluorescence microscopy
12.3.2 Samples for flow cytometry
12.3.3 Samples for environmental measurements and molecular diversity of viruses
12.4 Tools and reagents [Go to Page]
12.4.1 Equipment and reagents for epifluorescence microscopy
12.4.2 Equipment and reagents for flow cytometry
12.4.3 Equipment and tools for molecular diversity of viruses
12.5 Processing and analysis of samples [Go to Page]
12.5.1 Enumeration of viruses by epifluorescence microscopy
12.5.2 Procedure for flow cytometry
12.5.3 Estimation of the molecular diversity of viruses
13 Survey of benthic microbes [Go to Page]
13.1 Principle
13.2 General provisions
13.3 Collection and preservation of the samples [Go to Page]
13.3.1 Sampling stations and sample collection
13.3.2 On-site sample process
13.3.3 Sample storage
13.4 Tools and reagents [Go to Page]
13.4.1 Equipment
13.4.2 Reagents
13.5 Processing and analysis of the samples [Go to Page]
13.5.1 Assessment of molecular diversities of benthic microbes
13.5.2 Benthic microbial abundance
14 Survey of benthic microalgae [Go to Page]
14.1 Principle
14.2 General provisions
14.3 Collection and preservation of the samples [Go to Page]
14.3.1 Sampling design
14.3.2 Sampling methods
14.3.3 Sample preservation and fixation
14.4 Tools and reagents
14.5 Processing and analysis of the samples [Go to Page]
14.5.1 Sample processing
14.5.2 Sample analysis
14.5.3 Abundance and biomass calculation
14.5.4 Cyst culture and identification
14.5.5 Fluorescence determination of demagnesium chlorophyll and chlorophyll a
14.5.6 Algal toxins determination
15 Survey of benthic protozoa [Go to Page]
15.1 Principle
15.2 General provisions
15.3 Collection and preservation of the samples [Go to Page]
15.3.1 Sampling equipment
15.3.2 Sediment core collection
15.3.3 Sediment layers
15.3.4 Sample fixation and preservation
15.3.5 Collection and treatment of living protozoa
15.4 Tools and reagents [Go to Page]
15.4.1 Equipment and reagents for silica sol centrifugation
15.4.2 Equipment and reagents of quantitative protargol staining
15.4.3 Equipment and reagents for molecular analysis
15.5 Processing and analysis of the samples [Go to Page]
15.5.1 Silica sol density centrifugation
15.5.2 Quantitative protargol stain (QPS)
15.5.3 Estimation of molecular diversity
16 Survey of metazoan meiobenthos [Go to Page]
16.1 Principle
16.2 General provisions
16.3 Collection and preservation of the samples [Go to Page]
16.3.1 Sampling in intertidal zones and shallow waters
16.3.2 Deep-sea sampling
16.3.3 Sample slicing
16.3.4 Sample fixation and preservation
16.3.5 Labelling
16.3.6 Record
16.4 Tools and reagents
16.5 Processing and analysis of the samples [Go to Page]
16.5.1 Quantification and taxa analysis
16.5.2 Molecular analysis of biodiversity
16.6 Organization of data [Go to Page]
16.6.1 Precision
16.6.2 Abundance
16.6.3 Biomass
16.6.4 Complete the report
16.6.5 Plotting data
Annex A (informative) Photographs of several groups of communities of interstitial biota
Annex B (informative) Several stratified sampling devices for the survey of interstitial biota
Annex C (informative) Tables for sample labelling and sampling record — Examples
Annex D (informative) Tables of microscopy records — Examples
Annex E (informative) Calculation of community parameters
Annex F (informative) Record sheet for sample treatment — Examples
Annex G (informative) Characteristics for the classification of coccoliths
Annex H (informative) Flow diagram for the enumeration of viruses by epifluorescence microscopy
Annex I (informative) Filter unit used in microbenthos survey
Annex J (informative) Technical flowcharts for the investigation of microbenthos
Annex K (informative) Solutions and regents
Bibliography [Go to Page]

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