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BS EN ISO 21149:2017 Cosmetics. Microbiology. Enumeration and detection of aerobic mesophilic bacteria >
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BS EN ISO 21149:2017 Cosmetics. Microbiology. Enumeration and detection of aerobic mesophilic bacteria, 2017
- National foreword
- European foreword
- Endorsement notice
- Foreword
- 1 Scope
- 2 Normative references
- 3 Terms and definitions
- 4 Principle
- 4.1 General
- 4.2 Plate count
- 4.3 Membrane filtration
- 4.4 Detection of bacteria by enrichment
- 5 Diluents, neutralizers and culture media
- 5.1 General
- 5.2 Neutralizing diluents and diluents
- 5.3 Diluent for the bacterial suspension (tryptone sodium chloride solution)
- 5.4 Culture media
- 6 Apparatus and glassware
- 7 Strains of microorganisms
- 8 Handling of cosmetic products and laboratory samples
- 9 Procedure
- 9.1 General recommendation
- 9.2 Preparation of the initial suspension
- 9.2.1 General
- 9.2.2 Water-miscible products
- 9.2.3 Water-immiscible products
- 9.3 Counting methods
- 9.3.1 Dilutions for counting methods
- 9.3.2 Plate-count methods
- 9.4 Enrichment
- 9.4.1 General
- 9.4.2 Incubation of the sample
- 10 Counting of colonies (plate counts and membrane filtration methods)
- 11 Detection of growth (enrichment method)
- 12 Expression of results
- 12.1 Method of calculation for plate count
- 12.2 Interpretation
- 12.3 Examples
- 12.4 Detection after enrichment
- 13 Neutralization of the antimicrobial properties of the product
- 13.1 General
- 13.2 Preparation of inoculum
- 13.3 Suitability of counting methods
- 13.3.1 Principle
- 13.3.2 Suitability test of the pour-plate method
- 13.3.3 Suitability of the surface spread method
- 13.3.4 Suitability of the membrane filtration method
- 13.4 Suitability of the detection method by enrichment
- 13.4.1 Procedure
- 13.4.2 Interpretation of results
- 13.5 Interpretation of suitability test results
- 14 Test report
- Annex A (informative) Other neutralizing diluents
- Annex B (informative) Other diluents
- Annex C (informative) Other culture media
- Annex D (informative) Neutralizers of antimicrobial activity of preservatives and rinsing liquids
- Bibliography [Go to Page]