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  • BSI
    PD ISO/TS 20224-1:2020 Molecular biomarker analysis. Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR - Bovine DNA detection method
    Edition: 2020
    $435.35
    / user per year

Description of PD ISO/TS 20224-1:2020 2020

This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of bovine-specific DNA derived from food and feed. It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix and can be applied to the detection of bovine material derived from cattle including taurine (Bos taurus) and zebu (Bos indicus). The assay also detects the species bison (Bison bison) and yak (Bos mutus).

The target sequence is a partial fragment of the bovine nuclear beta actin gene in chromosome 25 (i.e. GenBank accession number NC_037352.1)[ 1][ 2][ 3], which is present as a single copy per haploid genome. The provided PCR assay for this target has an absolute limit of detection of five copies per reaction, with ? 95 % replicability at this concentration (LOD95 %).



Standard NumberPD ISO/TS 20224-1:2020
TitleMolecular biomarker analysis. Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR - Bovine DNA detection method
StatusUnder Review
Publication Date2020-08-13
Identical National Standard OfISO/TS 20224-1
DescriptorsBovine animals, Cattle, DNA, Biological analysis and testing, Food
ICS67.050 General methods of tests and analysis for food products
CommitteeAW/275
ISBN978 0 539 04892 6
PublisherBSI
Pages26

About BSI

BSI Group, also known as the British Standards Institution is the national standards body of the United Kingdom. BSI produces technical standards on a wide range of products and services and also supplies certification and standards-related services to businesses.
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