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  • BSI
    PD CEN/TS 15633-2:2013 Foodstuffs. Detection of food allergens by immunological methods - Quantitative determination of hazelnut with an enzyme immunoassay using monoclonal antibodies and bicinchoninic acid-protein detection
    Edition: 2013
    $442.60
    / user per year

Description of PD CEN/TS 15633-2:2013 2013

This Technical Specification specifies an enzyme linked immunsorbent assay (ELISA) method for the determination of hazelnut from food samples. In the ELISA the antibodies bind to hazelnut proteins from the food sample. The result of the ELISA is given in mg hazelnut/kg (ppm) because the calibrators consist of an extract of whole hazelnut.

Matrices like cereals, ice cream, cookies, chocolate, sausage, cottage cheese, yogurt and salad dressing were validated by spiking experiments with a carboxymethylcellulose-suspension containing hazelnut paste [2].

The monoclonal antibodies, raised against the whole aqueous extract of hazelnut, detect proteins with approximate molecular weights of 14 kDa, 18 kDa, and 42 kDa. The antibodies detect the major thermostable allergen Cor a9 (11S storage protein). Both antibodies were evaluated by western blots with partially purified hazelnut extracts and purified allergenic proteins.

The ELISA test method is commercially available1). The performance has been validated by an in house validation performed by the manufacturer. All parameters of interest are indicated.

In addition, the ELISA was successfully validated by a collaborative study in order to determine the interlaboratory reproducibility. This ring trial was organised by the working group established by the Federal Office of Consumer Protection and Food Safety (BVL) for the execution of § 64 of the German Food and Feed Code (LFGB) for the determination of hazelnut content in dark chocolate. Fourteen German laboratories participated in this collaborative study.



Standard NumberPD CEN/TS 15633-2:2013
TitleFoodstuffs. Detection of food allergens by immunological methods - Quantitative determination of hazelnut with an enzyme immunoassay using monoclonal antibodies and bicinchoninic acid-protein detection
StatusUnder Review
Publication Date2013-04-30
Identical National Standard OfCEN/TS 15633-2:2013
DescriptorsBiochemical methods of analysis, Food products, Proteins, Allergies, Immunoassay, Food testing, Biochemistry, Qualitative analysis, Antibodies
ICS67.050 General methods of tests and analysis for food products
CommitteeAW/275
ISBN978 0 580 75409 8
PublisherBSI
Pages36

About BSI

BSI Group, also known as the British Standards Institution is the national standards body of the United Kingdom. BSI produces technical standards on a wide range of products and services and also supplies certification and standards-related services to businesses.
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