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  • BSI
    BS EN ISO 11063:2013 Soil quality. Method to directly extract DNA from soil samples
    Edition: 2013
    $442.60
    / user per year

Description of BS EN ISO 11063:2013 2013

This International Standard specifies a method for direct extraction of DNA from soil samples to analyse the global structure and the abundance of soil bacterial communities using PCR-based technologies. This method is mainly dedicated to agricultural and forest soils. This method can possibly not be suitable for soils rich in organic matter (e.g. peat soils) or soils heavily polluted with organic pollutants or heavy metals.

The direct extraction of DNA from soil samples provides unique insight into the richness and structure of microbial communities which are key parameters to estimate the biodiversity of soil microbiota. Molecular approaches based on PCR (polymerase chain reaction) amplification of soil DNA constitute a promising domain and can contribute in the near future to the development of routine tools to monitor the microbiota of soil environments.

Users of the method ought to be aware that although soil submitted to the DNA extraction procedure is sieved thoroughly (2 mm mesh, procedure described in 5.1), plant residues can still remain in soil samples and, as a result, traces of plant DNA can contaminate the soil DNA extract.



Standard NumberBS EN ISO 11063:2013
TitleSoil quality. Method to directly extract DNA from soil samples
StatusWithdrawn
Publication Date2013-11-30
Withdrawn Date2020-10-20
Identical National Standard OfEN ISO 11063 (ISO 11063:2012) AS, ISO 11063:2012
DescriptorsData sampling, Soils, Information exchange, Quality
ICS13.080.30 Biological properties of soils
CommitteeEH/4
ISBN978 0 580 79339 4
PublisherBSI
Pages34

About BSI

BSI Group, also known as the British Standards Institution is the national standards body of the United Kingdom. BSI produces technical standards on a wide range of products and services and also supplies certification and standards-related services to businesses.
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