ASTM E1295-01(2006)
Historical Standard: ASTM E1295-01(2006) Standard Guide for Conducting Three-Brood, Renewal Toxicity Tests with Ceriodaphnia dubia
SUPERSEDED (see Active link, below)
ASTM E1295
1. Scope
1.1 This guide describes procedures for obtaining data concerning the adverse effects of an effluent or a test material (added to dilution water, but not to food) on Ceriodaphnia dubia Richard 1894, during continuous exposure throughout a portion of the organism's life. These procedures should also be useful for conducting life cycle toxicity tests with other Cladocera (Guide E 1193), although modifications will be necessary.
1.2 These procedures are applicable to most chemicals, either individually or in formulations, commercial products, or known mixtures, that can be measured accurately at the necessary concentrations in water. With appropriate modifications these procedures can be used to conduct tests on temperature, dissolved oxygen, pH, and on such materials as aqueous effluents (see also Guide E 1192), leachates, oils, particulate matter, sediments (see also Guide E 1383), and surface waters. Renewal tests might not be applicable to materials that have high oxygen demand, are highly volatile, are rapidly biologically or chemically transformed, or sorb to test chambers. If the concentration of dissolved oxygen falls below 4 mg/L or the concentration of test material decreases by more than 20 % in test solution(s) between renewals, more frequent renewals might be necessary.
1.3 Other modifications of these procedures might be justified by special needs or circumstances. Results of tests conducted using unusual procedures are not likely to be comparable to results of many other tests. Comparisons of results obtained using modified and unmodified versions of these procedures might provide useful information on new concepts and procedures for conducting three-brood toxicity tests with C. dubia.
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific hazard statements are given in Section 8.
1.4 This guide is arranged as follows:
| Section | |
| Referenced Documents | 2 |
| Terminology | 3 |
| Summary of Guide | 4 |
| Significance and Use | 5 |
| Apparatus | 6 |
| Facilities | 6.1 |
| Construction Materials | 6.2 |
| Test Chambers | 6.3 |
| Cleaning | 6.4 |
| Reagents and Materials | 7 |
| Hazards | 8 |
| Dilution Water | 9 |
| Requirements | 9.1 |
| Source | 9.2 |
| Treatment | 9.3 |
| Characterization | 9.4 |
| Test Material | 10 |
| General | 10.1 |
| Stock Solution | 10.2 |
| Effluent | 10.3 |
| Test Concentration(s) | 10.4 |
| Collection | 10.5 |
| Sample Containers | 10.6 |
| Preservation | 10.7 |
| Treatment | 10.8 |
| Test Organisms | 11 |
| Species | 11.1 |
| Age | 11.2 |
| Source | 11.3 |
| Brood Stock | 11.4 |
| Food | 11.5 |
| Handling | 11.6 |
| Quality | 11.7 |
| Procedure | 12 |
| Demonstration of Feasibility | 12.1 |
| Experimental Design | 12.2 |
| Dissolved Oxygen | 12.3 |
| Temperature | 12.4 |
| Preparing Test Solutions | 12.5 |
| Conditioning Test Chambers | 12.6 |
| Beginning a Test | 12.7 |
| Renewing Test Solutions | 12.8 |
| Duration of Test | 12.9 |
| Biological Data | 12.10 |
| Other Measurements | 12.11 |
| Analytical Methodology | 13 |
| Acceptability of Test | 14 |
| Calculation | 15 |
| Report | 16 |
| Appendixes | |
| Food | X1 |
| Culture Techniques | X2 |
| Test Chambers | X3 |
| Statistical Guidance | X4 |
2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.
ASTM Standards
D1193 Specification for Reagent Water
D3978 Practice for Algal Growth Potential Testing with Pseudokirchneriella subcapitata
E729 Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
E943 Terminology Relating to Biological Effects and Environmental Fate
E1023 Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses
E1192 Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians
E1193 Guide for Conducting Daphnia magna Life-Cycle Toxicity Tests
E1383 Guide for Conducting Sediment Toxicity Tests with Freshwater Invertebrates
E1706 Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Freshwater Invertebrates
IEEE/ASTM SI 10 American National Standard for Use of the International System of Units (SI): The Modern Metric System
Keywords
Ceriodaphnia dubia ; Cladocera ; effluent; life cycle; test material; three-brood toxicity test;
ICS Code
ICS Number Code 07.100.20 (Microbiology of water)
DOI: 10.1520/E1295-01R06
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