1. Scope

1.1 This guide describes procedures for obtaining data concerning the adverse effects of an effluent or a test material (added to dilution water, but not to food) on Ceriodaphnia dubia Richard 1894, during continuous exposure throughout a portion of the organism's life. These procedures should also be useful for conducting life cycle toxicity tests with other Cladocera (Guide E 1193), although modifications will be necessary.

1.2 These procedures are applicable to most chemicals, either individually or in formulations, commercial products, or known mixtures, that can be measured accurately at the necessary concentrations in water. With appropriate modifications these procedures can be used to conduct tests on temperature, dissolved oxygen, pH, and on such materials as aqueous effluents (see also Guide E 1192), leachates, oils, particulate matter, sediments (see also Guide E 1383), and surface waters. Renewal tests might not be applicable to materials that have high oxygen demand, are highly volatile, are rapidly biologically or chemically transformed, or sorb to test chambers. If the concentration of dissolved oxygen falls below 4 mg/L or the concentration of test material decreases by more than 20 % in test solution(s) between renewals, more frequent renewals might be necessary.

1.3 Other modifications of these procedures might be justified by special needs or circumstances. Results of tests conducted using unusual procedures are not likely to be comparable to results of many other tests. Comparisons of results obtained using modified and unmodified versions of these procedures might provide useful information on new concepts and procedures for conducting three-brood toxicity tests with C. dubia.

1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific hazard statements are given in Section 8.

1.4 This guide is arranged as follows:

2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

ASTM Standards

D1193 Specification for Reagent Water

D3978 Practice for Algal Growth Potential Testing with Pseudokirchneriella subcapitata

E729 Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians

E943 Terminology Relating to Biological Effects and Environmental Fate

E1023 Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses

E1192 Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians

E1193 Guide for Conducting Daphnia magna Life-Cycle Toxicity Tests

E1383 Guide for Conducting Sediment Toxicity Tests with Freshwater Invertebrates

E1706 Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Freshwater Invertebrates

IEEE/ASTM SI 10 American National Standard for Use of the International System of Units (SI): The Modern Metric System

Keywords

Ceriodaphnia dubia ; Cladocera ; effluent; life cycle; test material; three-brood toxicity test;

ICS Code

ICS Number Code 07.100.20 (Microbiology of water)

DOI: 10.1520/E1295-01

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