ASTM-D8375, 2023 - MADCAD.com

ASTM

D8375-23 Standard Test Method for Determination of Cannabinoid Concentration in Dried Cannabis and Hemp Raw Materials using Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Edition: 2023

$144.00
Unlimited Users per year

Description of ASTM-D8375 2023

ASTM D8375-23

Active Standard: Standard Test Method for Determination of Cannabinoid Concentration in Dried Cannabis and Hemp Raw Materials using Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

ASTM D8375

Scope

1.1 This test method allows for the concentration determination of the cannabinoids listed in Table 1, and shall apply to any dried raw material from a cannabis plant (Note 1, Note 2) regardless of the type of cannabis plant from which it was derived.2 For the sake of brevity, the term “cannabis” shall be used from now on to refer to any type of cannabis plant including those that can be classified as hemp. The procedure includes sub-sampling a ground, homogeneous sample, extraction with methanol:water (80:20, v:v),3^,4 dilution in methanol and analysis by liquid chromatography tandem mass spectrometry (LC-MS/MS). The method allows for a wide-range of sample concentrations to be determined by using a 1000-fold calibration range and the option to perform multiple levels of sample dilution. The calibration curve is prepared in methanol over a range of 10 ng/mL to 10 000 ng/mL for all seventeen cannabinoids, or a subset of cannabinoids if desired, while the sample extracts are diluted in methanol into the calibration range.3^,4^,5 For example, a 1/500 dilution of sample extracts allows concentration determination over a range of 0.5 mg/g to 500 mg/g in cannabis. The method was validated with quality control samples prepared in methanol, a candidate certified reference material (CRM), and repeat extraction and analysis of cannabinoid samples.3

Note 1: For this test method, dried raw material from a cannabis plant includes one or more of inflorescence, leaves, or stems.

Note 2: Certain jurisdictions or regulations may require specific parts of the plant to be included or excluded for analysis and those regulations will take precedence for the selection of plant parts.

1.2 Units—The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.3 List of Measurable Analytes—See Table 1.

1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.

1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

Keywords

analyses; cannabinoids; cannabis; hemp; laboratory; LC-MS/MS

QC SampleQC-LLOQ(ng/mL)QC-1(ng/mL)QC-2(ng/mL)QC-3(ng/mL)HempCRM(mg/g)Cannabis CRM(mg/g)HEMPRRSRecoveryCannabisRRSRecovery1030150080000.31848.9(%)(%)Batch-1n666666---Av.8.4127.8147474280.27950.3--Precision (%)0.7 %1.5 %0.8 %0.2 %0.5 %0.9 %--Accuracy (%)84.1 %92.5 %98.3 %92.8 %87.8 %103.0 %--Batch-2n666666---Av.9.1729.2152976440.27846.0--Precision (%)1.0 %1.3 %0.6 %0.8 %0.8 %%--Accuracy (%)91.7 %97.5 %101.9 %95.6 %87.3 %94.0 %--Batch-3n6666661-Av.9.0027.6150373680.27546.3--Precision (%)1.1 %0.6 %0.5 %0.3 %0.9 %0.7 %--Accuracy (%)90.0 %92.1 %100.2 %92.1 %86.4 %94.8 %95.6 %-QC sample Inter-Batch Statsn181818181818--Av.8.8628.2150274800.2847.6--Precision (%)3.9 %2.9 %1.6 %1.7 %1.0 %5.0 %--Accuracy (%)88.6 %94.0 %100.1 %93.5 %87.2 %97.3 %--

QC SampleQC-LLOQ(ng/mL)QC-1(ng/mL)QC-2(ng/mL)QC-3(ng/mL)HempCRM(mg/g)Cannabis CRM(mg/g)HEMPRRSRecoveryCannabisRRSRecovery1030150080000.979124(%)(%)Batch-1n666666--Av.8.8328.1146774371.01119--Precision (%)2.2 %1.3 %0.3 %0.8 %1.0 %1.2 %--Accuracy (%)88.3 %93.6 %97.8 %93.0 %102.8 %95.9 %--Batch-2n666666--Av.9.0729.1150775640.961106--Precision (%)1.0 %0.9 %0.4 %1.0 %1.2 %3.0 %--Accuracy (%)90.7 %97.0 %100.5 %94.6 %98.1 %85.3 %--Batch-3n666666--Av.9.127.3152073580.970111--Precision (%)2.2 %1.7 %0.6 %0.8 %1.4 %0.4 %--Accuracy (%)91.3 %91.0 %101.3 %92.0 %99.1 %89.5 %--QC sample Inter-Batch Statsn181818181818--Av.9.0128.2149874530.979112--Precision (%)2.3 %3.0 %1.6 %1.4 %2.4 %5.3 %--Accuracy (%)90.1 %93.8 %99.9 %93.2 %100.0 %90.2 %--

QC SampleQC-LLOQ(ng/mL)QC-1(ng/mL)QC-2(ng/mL)QC-3(ng/mL)HempCRM(mg/g)Cannabis CRM(mg/g)HEMPRRSRecoveryCannabisRRSRecovery1030150080005.418.33(%)(%)Batch-1n666666--Av.8.927.8146576345.158.60--Precision (%)3.1 %2.3 %0.5 %1.2 %1.2 %1.9 %--Accuracy (%)88.6 %92.7 %97.7 %95.4 %95.3 %103.2 %--Batch-2n666666--Av.9.428.8149176515.077.59--Precision (%)5.1 %1.2 %0.6 %1.0 %1.5 %3.8 %--Accuracy (%)94.2 %96.0 %99.4 %95.6 %93.7 %91.2 %--Batch-3n666666--Av.9.227.5149474835.047.97--Precision (%)4.6 %1.1 %0.9 %1.5 %1.1 %2.1 %--Accuracy (%)92.5 %91.8 %99.6 %93.5 %93.2 %95.7 %--QC sample Inter-Batch Statsn181818181818Av.9.1828.1148375895.098.06--Precision (%)4.9 %2.5 %1.1 %1.6 %1.5 %5.9 %--Accuracy (%)91.8 %93.5 %98.9 %94.9 %94.0 %96.7 %--

QC SampleQC-LLOQ(ng/mL)QC-1(ng/mL)QC-2(ng/mL)QC-3(ng/mL)HempCRM(mg/g)Cannabis CRM(mg/g)HEMPRRSRecoveryCannabisRRSRecovery10301500800014.623.0(%)(%)Batch-1n666666--Av.9.127.61462758413.523.5--Precision (%)3.1 %2.8 %1.4 %1.7 %2.0 %1.8 %--Accuracy (%)90.8 %91.9 %97.4 %94.8 %92.3 %102.1 %--Batch-2n666666--Av.9.228.71500768413.420.2--Precision (%)2.9 %2.2 %0.9 %1.0 %1.7 %4.0 %--Accuracy (%)92.1 %95.6 %100.0 %96.1 %91.5 %87.7 %--Batch-3n666666--Av.9.227.71529742013.422.2--Precision (%)2.5 %2.8 %0.7 %1.4 %2.2 %1.6 %--Accuracy (%)91.8 %92.4 %101.9 %92.8 %92.0 %96.4 %--QC sample Inter-Batch Statsn181818181818--Av.9.1628.01497756313.421.9--Precision (%)2.7 %3.1 %2.1 %2.0 %1.9 %6.8 %--Accuracy (%)91.6 %93.3 %99.8 %94.5 %91.9 %95.4 %--

QC SampleQC-LLOQ(ng/mL)QC-1(ng/mL)QC-2(ng/mL)QC-3(ng/mL)HempCRM(mg/g)Cannabis CRM(mg/g)HEMPRRSRecoveryCannabisRRSRecovery1030150080000.0482.14(%)(%)Batch-1n666666--Av.9.027.6143175720.03831.70--Precision (%)2.0 %1.2 %0.8 %0.5 %2.9 %0.8 %--Accuracy (%)90.5 %92.1 %95.4 %94.6 %80.1 %95.5 %--Batch-2n666666--Av.9.528.7148177630.03821.59--Precision (%)1.6 %1.4 %1.2 %1.1 %1.9 %1.9 %--Accuracy (%)95.4 %95.7 %98.7 %97.0 %80.0 %89.6 %--Batch-3n6666661-Av.9.026.8149875620.03831.60--Precision (%)2.2 %1.5 %1.1 %0.7 %1.2 %1.8 %--Accuracy (%)89.7 %89.4 %99.8 %94.5 %80.2 %90.0 %102.1-QC sample Inter-Batch Statsn181818181818--Av.9.1827.7147076320.0381.63--Precision (%)3.4 %3.1 %2.2 %1.5 %2.0 %3.3 %--Accuracy (%)91.8 %92.4 %98.0 %95.4 %80.1 %91.7 %--

QC SampleQC-LLOQ(ng/mL)QC-1(ng/mL)QC-2(ng/mL)QC-3(ng/mL)HempCRM(mg/g)Cannabis CRM(mg/g)HEMPRRSRecoveryCannabisRRSRecovery1030150080000.1173.89(%)(%)Batch-1n666666--Av.8.527.9146473920.1103.97--Precision (%)0.9 %1.0 %0.7 %0.9 %1.5 %1.0 %--Accuracy (%)84.9 %92.9 %97.6 %92.4 %93.9 %102.1 %--Batch-2n666666--Av.9.329.2150974870.1063.53--Precision (%)2.1 %1.1 %0.6 %0.8 %1.5 %3.3 %--Accuracy (%)93.2 %97.4 %100.6 %93.6 %90.7 %90.6 %--Batch-3n6666661-Av.8.927.6151874260.1073.76--Precision (%)2.5 %1.4 %0.4 %0.6 %0.9 %0.7 %--Accuracy (%)89.4 %91.9 %101.2 %92.8 %91.6 %96.5 %103.5-QC sample Inter-Batch Statsn181818181818--Av.8.9128.2149774350.1083.75--Precision (%)4.3 %2.9 %1.7 %0.9 %2.0 %5.3 %--Accuracy (%)89.1 %94.1 %99.8 %92.9 %92.1 %96.4 %--

QC SampleQC-LLOQ(ng/mL)QC-1(ng/mL)QC-2(ng/mL)QC-3(ng/mL)HempCRM(mg/g)Cannabis CRM(mg/g)HEMPRRSRecoveryCannabisRRSRecovery103015008000--(%)(%)Batch-1n6666----Av.9.027.514177617----Precision (%)3.9 %1.8 %0.8 %0.7 %----Accuracy (%)89.9 %91.5 %94.5 %95.2 %----Batch-2n6666----Av.9.728.414727775----Precision (%)1.7 %1.4 %1.1 %0.9 %----Accuracy (%)97.1 %94.6 %98.1 %97.2 %----Batch-3n6666--11Av.9.226.714747643----Precision (%)3.7 %1.8 %1.2 %0.8 %----Accuracy (%)92.4 %89.2 %98.3 %95.5 %--98.497.0QC sample Inter-Batch Statsn18181818----Av.9.3227.514547678----Precision (%)4.5 %3.0 %2.1 %1.2 %----Accuracy (%)93.2 %91.8 %97.0 %96.0 %----

QC SampleQC-LLOQ(ng/mL)QC-1(ng/mL)QC-2(ng/mL)QC-3(ng/mL)HempCRM(mg/g)Cannabis CRM(mg/g)HEMPRRSRecoveryCannabisRRSRecovery103015008000--(%)(%)Batch-1n6666----Av.8.528.914977238----Precision (%)3.1 %0.9 %1.2 %1.3 %----Accuracy (%)85.5 %96.2 %99.8 %90.5 %----Batch-2n6666----Av.9.230.315637395----Precision (%)1.9 %2.0 %0.9 %1.1 %----Accuracy (%)92.0 %101.0 %104.2 %92.4 %----Batch-3n6666--11Av.8.928.715627203----Precision (%)1.9 %0.6 %0.5 %0.7 %----Accuracy (%)88.8 %95.7 %104.1 %90.0 %--102.5102.5QC sample Inter-Batch Statsn18181818----Av.8.8829.315417278----Precision (%)3.8 %2.8 %2.2 %1.5 %----Accuracy (%)88.8 %97.7 %102.7 %91.0 %----

QC SampleQC-LLOQ(ng/mL)QC-1(ng/mL)QC-2(ng/mL)QC-3(ng/mL)HempCRM(mg/g)Cannabis CRM(mg/g)HEMPRRSRecoveryCannabisRRSRecovery1030150080000.4903.39(%)(%)Batch-1n666666--Av.8.927.1142675750.4613.70--Precision (%)2.3 %2.0 %0.7 %0.6 %1.1 %0.9 %--Accuracy (%)88.6 %90.5 %95.1 %94.7 %94.0 %109.2 %--Batch-2n666666--Av.9.628.7148777670.4533.41--Precision (%)1.4 %1.0 %0.3 %0.8 %1.7 %4.7 %--Accuracy (%)95.8 %95.5 %99.1 %97.1 %92.5 %100.6 %--Batch-3n6666661-Av.9.327.4148975330.4593.30--Precision (%)1.5 %0.9 %0.5 %0.3 %1.0 %0.4 %--Accuracy (%)93.4 %91.5 %99.3 %94.2 %93.6 %97.4 %96.2-QC sample Inter-Batch Statsn181818181818--Av.9.2627.7146776250.4583.47--Precision (%)3.7 %2.7 %2.1 %1.5 %1.4 %5.6 %--Accuracy (%)92.6 %92.5 %97.8 %95.3 %93.4 %102.4 %--

QC SampleQC-LLOQ(ng/mL)QC-1(ng/mL)QC-2(ng/mL)QC-3(ng/mL)HempCRM(mg/g)Cannabis CRM(mg/g)HEMPRRSRecoveryCannabisRRSRecovery1030150080000.3501.84(%)(%)Batch-1n666666--Av.8.728.7148874710.3652.11--Precision (%)4.4 %2.5 %0.8 %1.2 %1.8 %3.1 %--Accuracy (%)87.4 %95.6 %99.2 %93.4 %104.2 %114.4 %--Batch-2n666666--Av.8.729.1151075650.3371.86--Precision (%)4.9 %2.7 %1.4 %0.8 %2.6 %4.5 %--Accuracy (%)87.4 %97.1 %100.7 %94.6 %96.2 %101.3 %--Batch-3n6666661-Av.9.028.3151373320.3401.88--Precision (%)3.6 %3.3 %0.9 %1.4 %1.8 %2.0 %--Accuracy (%)90.2 %94.3 %100.9 %91.7 %97.3 %101.9 %103.8-QC sample Inter-Batch Statsn181818181818--Av.8.8328.7150474560.3471.95--Precision (%)4.3 %2.9 %1.3 %1.7 %4.1 %6.7 %--Accuracy (%)88.3 %95.7 %100.2 %93.2 %99.2 %105.9 %--

QC SampleQC-LLOQ(ng/mL)QC-1(ng/mL)QC-2(ng/mL)QC-3(ng/mL)HempCRM(mg/g)Cannabis CRM(mg/g)HEMPRRSRecoveryCannabisRRSRecovery103015008000--(%)(%)Batch-1n6666----Av.8.627.014477629----Precision (%)2.7 %1.0 %1.1 %0.5 %----Accuracy (%)85.7 %90.1 %96.5 %95.4 %----Batch-2n6666----Av.9.628.814727755----Precision (%)4.8 %2.4 %1.6 %1.3 %----Accuracy (%)96.3 %96.0 %98.1 %96.9 %----Batch-3n6666--11Av.9.227.114837395----Precision (%)3.8 %1.4 %1.5 %1.0 %----Accuracy (%)92.0 %90.5 %98.9 %92.4 %--100.799.9QC sample Inter-Batch Statsn18181818----Av.9.1327.714677593----Precision (%)6.1 %3.4 %1.7 %2.2 %----Accuracy (%)91.3 %92.2 %97.8 %94.9 %----

QC SampleQC-LLOQ(ng/mL)QC-1(ng/mL)QC-2(ng/mL)QC-3(ng/mL)HempCRM(mg/g)Cannabis CRM(mg/g)HEMPRRSRecoveryCannabisRRSRecovery1030150080000.3251.18(%)(%)Batch-1n666666--Av.9.027.1141377560.2761.03--Precision (%)3.4 %2.1 %0.9 %0.6 %1.6 %3.0 %--Accuracy (%)89.6 %90.3 %94.2 %97.0 %85.1 %100.7 %--Batch-2n666666--Av.9.328.9145778780.2681.04--Precision (%)3.4 %1.7 %0.9 %1.1 %0.8 %1.9 %--Accuracy (%)92.7 %96.5 %97.1 %98.5 %82.5 %101.9 %--Batch-3n66666611Av.9.627.3143576300.2681.03--Precision (%)4.2 %1.9 %0.7 %0.5 %1.4 %1.1 %--Accuracy (%)96.2 %91.1 %95.7 %95.4 %82.5 %101.4 %93.089.4QC sample Inter-Batch Statsn181818181818--Av.9.2927.8143577550.2711.03--Precision (%)4.6 %3.5 %1.5 %1.5 %1.9 %2.0 %--Accuracy (%)92.9 %92.6 %95.7 %96.9 %83.4 %101.3 %--

QC SampleQC-LLOQ(ng/mL)QC-1(ng/mL)QC-2(ng/mL)QC-3(ng/mL)HempCRM(mg/g)Cannabis CRM(mg/g)HEMPRRSRecoveryCannabisRRSRecovery1030150080000.4481.67(%)(%)Batch-1n666666--Av.8.927.8144876120.4001.70--Precision (%)4.1 %3.5 %2.0 %0.8 %1.4 %2.7 %--Accuracy (%)89.5 %92.5 %96.5 %95.1 %89.4 %101.6 %--Batch-2n666666--Av.9.729.4146877560.3891.54--Precision (%)2.6 %2.3 %1.0 %1.7 %1.6 %1.9 %--Accuracy (%)97.3 %98.1 %97.9 %96.9 %86.8 %91.9 %--Batch-3n66666611Av.9.127.3147976010.3851.60--Precision (%)5.9 %1.5 %2.1 %0.5 %2.5 %1.6 %--Accuracy (%)91.4 %91.0 %98.6 %95.0 %86.0 %95.8 %96.887.8QC sample Inter-Batch Statsn181818181818--Av.9.2728.2146576560.3921.61--Precision (%)5.5 %4.1 %1.9 %1.4 %2.4 %4.7 %--Accuracy (%)92.7 %93.9 %97.7 %95.7 %87.4 %96.4 %--

QC SampleQC-LLOQ(ng/mL)QC-1(ng/mL)QC-2(ng/mL)QC-3(ng/mL)HempCRM(mg/g)Cannabis CRM(mg/g)HEMPRRSRecoveryCannabisRRSRecovery1030150080000.01430.303(%)(%)Batch-1n666666--Av.8.728.1148873710.01160.224--Precision (%)2.6 %2.3 %1.1 %0.5 %4.9 %0.8 %--Accuracy (%)86.6 %93.5 %99.2 %92.1 %81.0 %88.7 %--Batch-2n666666--Av.9.329.1154875790.01170.213--Precision (%)4.2 %1.3 %1.2 %1.1 %5.4 %1.0 %--Accuracy (%)92.9 %96.8 %103.2 %94.7 %81.9 %84.5 %--Batch-3n66666611Av.9.427.7152373390.01190.211--Precision (%)4.4 %2.4 %0.7 %0.6 %4.3 %0.5 %--Accuracy (%)94.0 %92.5 %101.5 %91.7 %83.5 %83.8 %99.1104.1QC sample Inter-Batch Statsn181818181818--Av.9.1228.3152074300.01170.216--Precision (%)5.2 %2.8 %1.9 %1.7 %4.8 %2.7 %--Accuracy (%)91.2 %94.3 %101.3 %92.9 %82.1 %85.7 %--

QC SampleQC-LLOQ(ng/mL)QC-1(ng/mL)QC-2(ng/mL)QC-3(ng/mL)HempCRM(mg/g)Cannabis CRM(mg/g)HEMPRRSRecoveryCannabisRRSRecovery1030150080000.07280.446(%)(%)Batch-1n666666--Av.8.727.5146774550.06860.423--Precision (%)1.2 %1.5 %0.6 %0.7 %1.6 %1.0 %--Accuracy (%)86.9 %91.6 %97.8 %93.2 %94.2 %94.7 %--Batch-2n666666--Av.9.228.9152175690.06750.405--Precision (%)2.9 %1.4 %0.9 %0.7 %1.9 %1.6 %--Accuracy (%)91.8 %96.2 %101.4 %94.6 %92.7 %90.7 %--Batch-3n66666611Av.9.227.8153573630.06950.411--Precision (%)1.9 %1.1 %0.6 %0.6 %1.7 %0.7 %--Accuracy (%)91.6 %92.8 %102.3 %92.0 %95.4 %92.1 %101.1102.5QC sample Inter-Batch Statsn181818181818--Av.9.0128.1150874620.06850.413--Precision (%)3.3 %2.5 %2.1 %1.3 %2.0 %2.2 %--Accuracy (%)90.1 %93.5 %100.5 %93.3 %94.1 %92.5 %--

QC SampleQC-LLOQ(ng/mL)QC-1 (ng/mL)QC-2(ng/mL)QC-3(ng/mL)HempCRM(mg/g)Cannabis CRM(mg/g)HEMPRRSRecoveryCannabisRRSRecovery1030150080000.1880.0415(%)(%)Batch-1n666666--Av.9.028.0147476480.1750.0389--Precision (%)3.0 %2.6 %1.5 %1.7 %1.9 %3.6 %--Accuracy (%)90.3 %93.5 %98.3 %95.6 %93.4 %93.7 %--Batch-2n666666--Av.9.228.8151777320.1700.0372--Precision (%)2.7 %2.0 %1.5 %0.9 %1.1 %2.7 %--Accuracy (%)92.3 %95.9 %101.1 %96.7 %90.2 %89.6 %--Batch-3n66666611Av.9.327.9152073610.1690.0368--Precision (%)3.9 %2.4 %0.6 %1.4 %0.9 %2.1 %--Accuracy (%)93.3 %93.0 %101.3 %92.0 %89.9 %88.7 %96.798.6QC sample Inter-Batch Statsn181818181818--Av.9.2028.2150475800.1710.04--Precision (%)3.4 %2.6 %1.8 %2.5 %2.2 %3.7 %--Accuracy (%)92.0 %94.1 %100.2 %94.8 %91.2 %90.7 %--

QC SampleQC-LLOQ(ng/mL)QC-1(ng/mL)QC-2(ng/mL)QC-3(ng/mL)HempCRM(mg/g)Cannabis CRM(mg/g)HEMPRRSRecoveryCannabisRRSRecovery1030150080000.7190.126(%)(%)Batch-1n666666--Av.8.827.5145375600.7140.111--Precision (%)2.1 %1.0 %1.6 %1.8 %1.8 %2.6 %--Accuracy (%)88.1 %91.5 %96.9 %94.5 %99.2 %88.3 %--Batch-2n666666--Av.9.128.5150376660.6950.108--Precision (%)2.1 %1.2 %1.3 %1.0 %0.7 %5.6 %--Accuracy (%)90.6 %94.9 %100.2 %95.8 %96.7 %86.1 %--Batch-3n66666611Av.9.228.1153474220.7110.112--Precision (%)2.0 %1.1 %1.2 %1.2 %2.1 %0.9 %--Accuracy (%)92.3 %93.5 %102.3 %92.8 %98.9 %88.6 %104.2100.0QC sample Inter-Batch Statsn181818181818--Av.9.0328.0149775490.7070.110--Precision (%)2.8 %1.8 %2.6 %1.9 %2.0 %3.6 %--Accuracy (%)90.3 %93.3 %99.8 %94.4 %98.3 %87.7 %--

QC SampleQC-LLOQ(ng/mL)QC-1(ng/mL)QC-2(ng/mL)QC-3(ng/mL)HempCRM(mg/g)CannabisCRM(mg/g)HEMPRRSRecoveryCannabisRRSRecovery1030150080000.0741-(%)(%)Batch-1n66666---Av.8.627.5146075310.0662---Precision (%)1.3 %1.4 %0.7 %0.7 %0.7 %---Accuracy (%)86.0 %91.8 %97.3 %94.1 %89.3 %---Batch-2n66666---Av.8.928.9151576480.0638---Precision (%)1.9 %1.1 %0.6 %0.7 %1.1 %---Accuracy (%)89.0 %96.3 %101.0 %95.6 %86.1 %---Batch-3n66666-11Av.9.027.2149074230.0637---Precision (%)1.7 %1.1 %0.6 %0.6 %0.8 %---Accuracy (%)90.2 %90.7 %99.3 %92.8 %86.0 %-96.894.7QC sample Inter-Batch Statsn1818181818---Av.8.8427.9148875340.0646---Precision (%)2.6 %2.9 %1.6 %1.4 %2.0 %---Accuracy (%)88.4 %92.9 %99.2 %94.2 %87.1 %---

QC SampleQC-LLOQ(ng/mL)QC-1(ng/mL)QC-2(ng/mL)QC-3(ng/mL)HempCRM(mg/g)CannabisCRM(mg/g)HEMPRRSRecoveryCannabisRRSRecovery1030150080000.187-(%)(%)Batch-1n66666---Av.8.627.6150877680.205---Precision (%)2.7 %2.5 %3.7 %3.2 %1.6 %---Accuracy (%)86.4 %92.1 %100.5 %97.1 %109.7 %---Batch-2n66666---Av.9.629.4150177150.193---Precision (%)2.3 %1.0 %1.6 %0.6 %1.3 %---Accuracy (%)95.9 %97.9 %100.0 %96.4 %103.1 %---Batch-3n66666-11Av.9.227.4151473690.197---Precision (%)3.3 %2.6 %2.4 %1.7 %1.9 %---Accuracy (%)92.4 %91.4 %100.9 %92.1 %105.4 %-101.291.3QC sample Inter-Batch Statsn1818181818---Av.9.1628.1150876170.198---Precision (%)5.1 %3.7 %2.6 %3.1 %3.1 %---Accuracy (%)91.6 %93.8 %100.5 %95.2 %106.1 %---

QC SampleQC-LLOQ(ng/mL)QC-1(ng/mL)QC-2(ng/mL)QC-3(ng/mL)HempCRM(mg/g)CannabisCRM(mg/g)HEMPRRSRecoveryCannabisRRSRecovery103015008000--(%)(%)Batch-1n6666----Av.8.727.914677500----Precision (%)1.5 %1.5 %0.8 %0.6 %----Accuracy (%)87.5 %93.0 %97.8 %93.8 %----Batch-2n6666----Av.9.229.315247667----Precision (%)2.4 %0.8 %0.7 %0.7 %----Accuracy (%)92.2 %97.7 %101.6 %95.8 %----Batch-3n6666--11Av.9.127.414947465----Precision (%)2.3 %0.9 %0.3 %0.6 %----Accuracy (%)91.0 %91.5 %99.6 %93.3 %--98.195.7QC sample Inter-Batch Statsn18181818----Av.9.0228.214957544----Precision (%)3.0 %3.1 %1.7 %1.3 %----Accuracy (%)90.2 %94.1 %99.7 %94.3 %----

IDTHCTHCACBDCBDACBGCBGACBNCBNACBCCBCATHCVTHCVACBDVCBDVACBLCBLAConc (mg/g)16.2651.44.3793.20.7213.150.1230.8450.4225.250.0510.4070.0200.428<0.0020.00914Repeatability (%)9.27.68.98.87.19.86.55.53.99.86.27.81.86.7N/AP7.8Int Precision (%)7.16.18.5 5.97.17.85.64.3 5.06.95.3 6.56.86.5N/AP8.0Conc (mg/g)226.926.018.167.60.4741.800.020.7621.464.080.2040.2100.07000.2820.00210.0218Repeatability (%)3.64.63.64.03.54.35.13.64.94.54.93.36.24.18.510.0Int Precision (%)4.43.94.0 3.74.24.14.25.2 5.75.94.6 3.74.94.510.09.3Conc (mg/g)36.921240.0100.2750.7262.180.1552.360.0920.9730.0340.569<0.0020.0024<0.002<0.002Repeatability (%)3.44.74.45.44.05.34.54.62.010.33.12.6N/AP5.4N/APN/APInt Precision (%)6.15.98.1 7.67.48.011.07.4 8.810.78.0 6.8N/AP11.9N/APN/APConc (mg/g)45.271660.007360.3611.053.830.0200.5150.1593.040.01330.279<0.002<0.002<0.002<0.002Repeatability (%)3.12.18.82.81.72.21.52.62.95.112.43.8N/APN/APN/APN/APInt Precision (%)8.98.112.8 6.57.28.17.48.7 5.57.011.0 7.1N/APN/APN/APN/APConc (mg/g)50.9174.899.261410.3911.290.0240.06430.6786.490.01030.05760.08301.38<0.002<0.002Repeatability (%)6.54.05.66.34.77.51.27.07.77.05.82.21.95.9N/APN/APInt Precision (%)5.23.85.5 4.43.85.43.65.1 5.45.54.4 2.23.64.8N/APN/APConc (mg/g)60.8035.169.311510.6362.530.0350.1130.7557.910.002670.01570.02260.3620.00790.0655Repeatability (%)2.63.23.41.63.63.13.94.52.75.45.63.35.24.43.63.7Int Precision (%)4.64.55.73.84.34.84.24.2 4.95.28.2 8.85.64.33.710.8Conc (mg/g)76.6913.90.008670.04620.0630.3800.1290.2510.2840.5510.04360.104<0.002<0.002<0.002<0.002Repeatability (%)4.84.86.88.15.34.67.67.23.97.86.06.1N/APN/APN/APN/APInt Precision (%)5.54.98.76.34.35.44.66.34.45.84.84.7N/APN/APN/APN/AP

;

ICS Code

ICS Number Code 11.120.99 (Other standards related to pharmaceutics)

DOI: 10.1520/D8375-23

The following editions for this book are also available...

Format	Year	Publisher	Type	Title				Annual Price
	2022	ASTM	Model Standard	D8375-22 Standard Test Method for Determination of Cannabinoid Concentration in Dried Cannabis and Hemp Raw Materials using Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)				$144.00	Buy
	2023	ASTM	Model Standard	D8375-23 Standard Test Method for Determination of Cannabinoid Concentration in Dried Cannabis and Hemp Raw Materials using Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS) (Redline)				$144.00	Buy

This book also exists in the following packages...

Year	Publisher	Title		Annual Price
VAR	ASTM	ASTM Volume 15.10 Packaging; Flexible Barrier Packaging	[+]	$1,335.14	Buy
2006	ASTM	F1249-06 Standard Test Method for Water Vapor Transmission Rate Through Plastic Film and Sheeting Using a Modulated Infrared Sensor
1995	ASTM	F1249-90(1995) Standard Test Method for Water Vapor Transmission Rate Through Plastic Film and Sheeting Using a Modulated Infrared Sensor
1998	ASTM	F904-98 Standard Test Method for Comparison of Bond Strength or Ply Adhesion of Similar Laminates Made from Flexible Materials
2003	ASTM	F904-98(2003) Standard Test Method for Comparison of Bond Strength or Ply Adhesion of Similar Laminates Made from Flexible Materials
2008	ASTM	F904-98(2008) Standard Test Method for Comparison of Bond Strength or Ply Adhesion of Similar Laminates Made from Flexible Materials
2009	ASTM	D1185-98a(2009) Standard Test Methods for Pallets and Related Structures Employed in Materials Handling and Shipping
2003	ASTM	D1596-97(2003) Standard Test Method for Dynamic Shock Cushioning Characteristics of Packaging Material
2004	ASTM	D1874-62(2004) Standard Specification for Water- or Solvent-Soluble Liquid Adhesives for Automatic Machine Sealing of Top Flaps of Fiberboard Shipping Cases
2006	ASTM	D1975-95(2006) Standard Test Method for Environmental Stress Crack Resistance of Plastic Injection Molded Open Head Pails
2007	ASTM	D1994-07 Standard Test Method for Determination of Acid Numbers of Hot-Melt Adhesives
		Showing 10 of 1410+ books in package. Click here to download full list of books.
VAR	ASTM	ASTM Section 15 - General Products, Chemical Specialties, and End Use Products	[+]	$9,043.85	Buy
2003	ASTM	A627-03 Standard Test Methods for Tool-Resisting Steel Bars, Flats, and Shapes for Detention and Correctional Facilities
1995	ASTM	A627-95 Standard Specification for Homogeneous Tool-Resisting Steel Bars for Security Applications
2004	ASTM	C1027-99(2004) Standard Test Method for Determining Visible Abrasion Resistance of Glazed Ceramic Tile
2006	ASTM	C1028-06 Standard Test Method for Determining the Static Coefficient of Friction of Ceramic Tile and Other Like Surfaces by the Horizontal Dynamometer Pull-Meter Method
2007	ASTM	C1028-07 Standard Test Method for Determining the Static Coefficient of Friction of Ceramic Tile and Other Like Surfaces by the Horizontal Dynamometer Pull-Meter Method
2006	ASTM	C1036-06 Standard Specification for Flat Glass
2004	ASTM	C1048-04 Standard Specification for Heat-Treated Flat Glassâ€”Kind HS, Kind FT Coated and Uncoated Glass
2000	ASTM	C1053-00 Standard Specification for Borosilicate Glass Pipe and Fittings for Drain, Waste, and Vent (DWV) Applications
2005	ASTM	C1053-00(2005) Standard Specification for Borosilicate Glass Pipe and Fittings for Drain, Waste, and Vent (DWV) Applications
2003	ASTM	C1054-03 Standard Practice for Pressing and Drying Refractory Plastic and Ramming Mix Specimens
		Showing 10 of 12920+ books in package. Click here to download full list of books.

Subscription Information

MADCAD.com ASTM Standards subscriptions are annual and access is unlimited concurrency based (number of people that can access the subscription at any given time) from single office location. For pricing on multiple office location ASTM Standards Subscriptions, please contact us at info@madcad.com or +1 800.798.9296.

Some features of MADCAD.com ASTM Standards Subscriptions are:

- Online access: With MADCAD.com’ s web based subscription service no downloads or installations are required. Access ASTM Standards from any browser on your computer, tablet or smart phone.

- Immediate Access: As soon as the transaction is completed, your ASTM Standards Subscription will be ready for access.

For any further information on MADCAD.com ASTM Standards Subscriptions, please contact us at info@madcad.com or +1 800.798.9296.

About ASTM

ASTM International, formerly known as the American Society for Testing and Materials (ASTM), is a globally recognized leader in the development and delivery of international voluntary consensus standards. Today, some 12,000 ASTM standards are used around the world to improve product quality, enhance safety, facilitate market access and trade, and build consumer confidence. ASTM’s leadership in international standards development is driven by the contributions of its members: more than 30,000 of the world’s top technical experts and business professionals representing 150 countries. Working in an open and transparent process and using ASTM’s advanced electronic infrastructure, ASTM members deliver the test methods, specifications, guides, and practices that support industries and governments worldwide.

Email *
Password *
Confirm *
First Name *
Last Name *
Title
Company Name *
Company Size *
Industry *
Address *
Address 2
City *
State *	Zip *
Country *
Phone *
How did you hear about MADCAD.com? *

Company*
Full name*
Email*
Phone

Full name*
Email*
Phone
Your selection

MADCAD.com Free Trial

Report To Us

Contact us for a custom quote

⨯To continue with accurate pricing,
please select your company type below:

⨯We need your input!

⨯ We need your input!

⨯We need your input!

⨯We need your input!

Cart Warning

Description of ASTM-D8375 2023

ASTM D8375-23

Active Standard: Standard Test Method for Determination of Cannabinoid Concentration in Dried Cannabis and Hemp Raw Materials using Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

ASTM D8375

The following editions for this book are also available...

This book also exists in the following packages...

Subscription Information

About ASTM

Username:
Password:

MADCAD.com Free Trial

Report To Us

Contact us for a custom quote

⨯To continue with accurate pricing, please select your company type below:

⨯We need your input!

⨯ We need your input!

⨯We need your input!

⨯We need your input!

Cart Warning

Description of ASTM-D8375 2023

ASTM D8375-23

Active Standard: Standard Test Method for Determination of Cannabinoid Concentration in Dried Cannabis and Hemp Raw Materials using Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

ASTM D8375

⨯To continue with accurate pricing,
please select your company type below: