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Description of ASTM-D7687 2023ASTM D7687-23Active Standard: Standard Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel and Fuel-associated Water With Sample Concentration by FiltrationASTM D7687Scope 1.1 This test method covers a protocol for capturing, extracting and quantifying the cellular adenosine triphosphate (cellular-ATP) content associated with microorganisms found in fuels and fuel-associated water. 1.2 The ATP is measured using a bioluminescence enzyme assay, whereby light is generated in amounts proportional to the concentration of cellular-ATP in the samples. The light is produced and measured quantitatively as relative light units (RLU) which are converted by comparison with an ATP standard, computation to pg ATP/mL and optional further transformation to Log10[pg ATP/mL]. 1.3 This test method is equally suitable for use as a laboratory or portable method. 1.4 This test method is limited to fuels with a nominal viscosity ≤75 cSt at test temperature. 1.5 This test method detects ATP concentrations in the range of 5.0 pg ATP/mL (≈0.699 log10[pg ATP/mL]) to 100 000 pg ATP/mL (≈5.000 log10[pg ATP/mL]) for 20 mL samples of fuel and 20 pg ATP/mL (≈1.301 log10[pg ATP/mL]) to 400 000 pg ATP/mL (≈5.602 log10[pg ATP/mL]) for 5 mL samples of fuel-associated water. Note 1: These ranges were calculated with the formula for calculating sample ATP in pg/mL provided in 12.1 based on the minimum recommended RLU for a 1 ng/mL ATP standard when using the reagents specified in Section 7 and the luminometer specified in 6.4 and corrected with a reagent-method blank as determined in Appendix X5. 1.6 Providing interferences can be overcome, bioluminescence is a reliable and proven method for qualifying and quantifying ATP. This test method does not differentiate between ATP from different sources, for example: from different types of microorganisms, such as bacteria and fungi. 1.7 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.8 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.9 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. Keywords adenosine triphosphate; ATP; bacteria; bioburden; biodeterioration; biodiesel; biofuels; biomass; cellular; cellular-ATP; diesel; fuel; fuel-oil; fungi; gasoline; microbial contamination; microbiology; microorganisms; ICS Code ICS Number Code 75.160.20 (Liquid fuels) DOI: 10.1520/D7687-23 The following editions for this book are also available...This book also exists in the following packages...Subscription InformationMADCAD.com ASTM Standards subscriptions are annual and access is unlimited concurrency based (number of people that can access the subscription at any given time) from single office location. For pricing on multiple office location ASTM Standards Subscriptions, please contact us at info@madcad.com or +1 800.798.9296.
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About ASTMASTM International, formerly known as the American Society for Testing and Materials (ASTM), is a globally recognized leader in the development and delivery of international voluntary consensus standards. Today, some 12,000 ASTM standards are used around the world to improve product quality, enhance safety, facilitate market access and trade, and build consumer confidence. ASTM’s leadership in international standards development is driven by the contributions of its members: more than 30,000 of the world’s top technical experts and business professionals representing 150 countries. Working in an open and transparent process and using ASTM’s advanced electronic infrastructure, ASTM members deliver the test methods, specifications, guides, and practices that support industries and governments worldwide. |
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