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Description of ASTM-D7463 2014ASTM D7463-14Historical Standard: Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures and Fuel Associated WaterASTM D7463Scope 1.1 This test method provides a protocol for capturing, extracting and quantifying the adenosine triphosphate (ATP) content associated with: 1.1.1 Microorganisms found in conventional liquid fuels with kinematic viscosities (at 40°C) of ≤ 8 mm2 · s–1 as described in Table X6.1, 1.1.2 Microorganisms found in fuel-associated bottom water, and 1.1.3 Extracellular (non-cellular) ATP present in the sample matrix. 1.2 The ATP is measured using a patented bioluminescence enzyme assay, whereby light is generated in amounts proportional to the concentration of ATP in the sample. The light is produced and measured quantitatively using dedicated ATP test pens2 and a dedicated luminometer2 and reported in (instrument specific) Relative Light Units. 1.3 This test method is equally suitable for use in the laboratory or field. 1.4 Although bioluminescence is a reliable and proven method for qualifying and quantifying ATP, this method does not differentiate between ATP from different sources, for example, from different types of microorganism such as bacteria or fungi. 1.5 For water or capture solution samples, the concentration range of ATP detectable by this test method is 1 × 10–11 M to 3 × 10–8 M which is equivalent to 1 × 10–14 moles/mL to 3 × 10–11 moles/mL for water samples or capture solution. Assuming testing on fuel phase is performed on a 500 mL volume of fuel the equivalent concentrations is fuel would be: 6 × 10–11 M to 2 × 10–14 M. 1.6 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Keywords adenosine triphosphate; ATP; bacteria; biocontamination; biodeterioration; biomass; fuel; fungi; microbe; microbiology ICS Code ICS Number Code 07.100.99 (Other standards related to microbiology) DOI: 10.1520/D7463-14 The following editions for this book are also available...
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About ASTMASTM International, formerly known as the American Society for Testing and Materials (ASTM), is a globally recognized leader in the development and delivery of international voluntary consensus standards. Today, some 12,000 ASTM standards are used around the world to improve product quality, enhance safety, facilitate market access and trade, and build consumer confidence. ASTM’s leadership in international standards development is driven by the contributions of its members: more than 30,000 of the world’s top technical experts and business professionals representing 150 countries. Working in an open and transparent process and using ASTM’s advanced electronic infrastructure, ASTM members deliver the test methods, specifications, guides, and practices that support industries and governments worldwide. |
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