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  • ASTM
    D6530-00(2013) Standard Test Method for Total Active Biomass in Cooling Tower Waters (Kool Kount Assay; KKA)
    Edition: 2013
    $103.58
    Unlimited Users per year

Description of ASTM-D6530 2013

ASTM D6530 - 00(2013)

Standard Test Method for Total Active Biomass in Cooling Tower Waters (Kool Kount Assay; KKA)

Active Standard ASTM D6530 | Developed by Subcommittee: D19.24

Book of Standards Volume: 11.02




ASTM D6530

Significance and Use

5.1 This test method is useful for rapid determination of viable active biomass concentrations in cooling tower waters. The efficiency of cooling towers is directly affected by the concentration of biomass in the cooling tower waters. As biomass concentrations increase, biofilm formation occurs resulting in a decrease in the efficiency of heat exchange in the tower. Current tests for monitoring the biomass concentration in cooling towers require at least 36 h for growth of the microorganisms on a solid agar surface for counting. Replication of microorganisms over the 36-h period before results are available creates an aqueous environment which is no longer represented by the data generated. Timely test results can assist in minimizing biocide addition to control biomass concentrations. Kool Kount provides data within hours to allow for more precise control of active biomass concentrations in the waters.

1. Scope

1.1 This test method covers the determination of viable active biomass in cooling tower water in the range from 10 2 to 10 8 cfu/mL (1). It is a semiquantitative test method.

1.2 This test method was used successfully with reagent water, physiologic saline, and cooling tower waters. It is the user's responsibility to ensure the validity of this test method for waters of untested matrices.

1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. For specific hazard statements, see Section 9.


2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

ASTM Standards

D1129 Terminology Relating to Water

D1192 Guide for Equipment for Sampling Water and Steam in Closed Conduits

D1193 Specification for Reagent Water

D3370 Practices for Sampling Water from Closed Conduits


Keywords

colorimetric bioassay; cooling tower water; triphenyl tetrazolium chloride; viable active biomass;


ICS Code

ICS Number Code 27.200 (Refrigerating technology)


DOI: 10.1520/D6530

ASTM International is a member of CrossRef.

ASTM D6530

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About ASTM

ASTM International, formerly known as the American Society for Testing and Materials (ASTM), is a globally recognized leader in the development and delivery of international voluntary consensus standards. Today, some 12,000 ASTM standards are used around the world to improve product quality, enhance safety, facilitate market access and trade, and build consumer confidence. ASTM’s leadership in international standards development is driven by the contributions of its members: more than 30,000 of the world’s top technical experts and business professionals representing 150 countries. Working in an open and transparent process and using ASTM’s advanced electronic infrastructure, ASTM members deliver the test methods, specifications, guides, and practices that support industries and governments worldwide.
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