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PD ISO/TS 21872-2:2020 Microbiology of the food chain. Horizontal method for the determination of Vibrio spp. - Enumeration of total and potentially enteropathogenic Vibrio parahaemolyticus in seafood using nucleic acid hybridization >
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PD ISO/TS 21872-2:2020 Microbiology of the food chain. Horizontal method for the determination of Vibrio spp. - Enumeration of total and potentially enteropathogenic Vibrio parahaemolyticus in seafood using nucleic acid hybridization, 2020
- undefined
- Foreword
- Introduction
- 1 Scope
- 2 Normative references
- 3 Terms and definitions
- 4 Principle
- 4.1 General
- 4.2 Plating
- 4.3 Preparation of membranes
- 4.4 Hybridization
- 4.5 Detection
- 5 Culture media and reagents
- 5.1 General
- 5.2 Hybridization reagents
- 5.2.1 Hybridization buffer
- 5.2.2 Digoxigenin-labelled oligoprobes
- 5.2.3 Control materials
- 6 Equipment and consumables
- 7 Sampling
- 8 Procedure
- 8.1 General
- 8.2 Preparation of the sample
- 8.3 Plating
- 8.4 Preparation of process control
- 8.5 Preparation of membranes
- 8.5.1 Colony lift
- 8.5.2 Cross-linking of DNA to the membrane
- 8.5.3 Proteinase K treatment
- 8.6 Hybridization
- 8.6.1 General
- 8.6.2 Hybridization of tdh and trh probes
- 8.6.3 Colorimetric detection
- 8.6.4 Enumeration of tdh and/or trh positive colonies
- 8.6.5 Isolation of colonies (optional)
- 8.6.6 Hybridization of toxR probe
- 8.6.7 Colorimetric detection
- 8.6.8 Enumeration of toxR positive colonies
- 9 Interpretation of the results
- 10 Expression of results
- 11 Test report
- Annex A (normative) Flow diagram for the enumeration of total and potentially enteropathogenic Vibrio parahaemolyticus in seafood using nucleic acid hybridization
- Annex B (normative) Culture media and reagents
- Annex C (informative) Hybridization of tdh, trh and toxR genes of V. parahaemolyticus
- Bibliography [Go to Page]
