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eLibrary > BS ISO 23305:2020 Fortified milk powders, infant formula and adult nutritionals. Determination of total biotin by liquid chromatography coupled with immunoaffinity column clean-up extraction >

BS ISO 23305:2020 Fortified milk powders, infant formula and adult nutritionals. Determination of total biotin by liquid chromatography coupled with immunoaffinity column clean-up extraction, 2020

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BS ISO 23305:2020 Fortified milk powders, infant formula and adult nutritionals. Determination of total biotin by liquid chromatography coupled with immunoaffinity column clean-up extraction, 2020

National foreword
Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents and materials [Go to Page]
5.1 General [Go to Page]
5.1.1 Laboratory reagent grade water.
5.1.2 Sodium dihydrogen phosphate dihydrate (CAS # 13472-35-0).
5.1.3 Disodium hydrogen phosphate dihydrate (CAS # 10028-24-7).
5.1.4 Sodium hydroxide (CAS #1310-73-2).
5.1.5 Methanol, HPLC grade (CAS # 67-56-1).
5.1.6 Acetonitrile, HPLC grade (CAS # 75-05-8).
5.1.7 Ortho-phosphoric acid, mass fraction w (H3PO4) = 85 % (CAS # 7664-38-2).
5.2 Reagent preparation
5.3 Standard preparation
5.4 Calculation of concentration
6 Apparatus [Go to Page]
6.10 Horizontal shaker.
6.18 Vortex mixer.
7 Procedure [Go to Page]
7.1 Sample preparation
7.2 Chromatography
7.3 Quality control
8 Calculations
9 Precision [Go to Page]
9.1 General
9.2 Repeatability
9.3 Reproducibility
10 Uncertainty of measurement
11 Limit of quantitation
Annex A (informative) Example chromatograms
Annex B (informative) Precision data
Annex C (informative) Comparison between this document and EN 15607
Bibliography [Go to Page]

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BS ISO 23305:2020 Fortified milk powders, infant formula and adult nutritionals. Determination of total biotin by liquid chromatography coupled with immunoaffinity column clean-up extraction, 2020

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