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eLibrary > BS ISO 21160:2018 Cigarettes. Determination of selected carbonyls in the mainstream smoke of cigarettes. Method using high performance liquid chromatography >

BS ISO 21160:2018 Cigarettes. Determination of selected carbonyls in the mainstream smoke of cigarettes. Method using high performance liquid chromatography, 2018

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BS ISO 21160:2018 Cigarettes. Determination of selected carbonyls in the mainstream smoke of cigarettes. Method using high performance liquid chromatography, 2018

National foreword
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Apparatus
6 Reagents
7 Preparation [Go to Page]
7.1 Preparation of glassware
7.2 Preparation of solutions
7.3 Preparation of standards [Go to Page]
7.3.1 HPLC calibration standards and working solutions
7.3.2 Carbonyl working standards
8 Sampling
9 Tobacco product preparation
10 Sample generation — Smoking of cigarettes [Go to Page]
10.1 General
10.2 Smoking machine setup
10.3 Smoking [Go to Page]
10.3.1 General
10.3.2 Linear smoking
10.3.3 Rotary smoking
11 Sample analysis [Go to Page]
11.1 Preparation of mainstream smoke extract solution
11.2 Reversed phase high performance liquid chromatography [Go to Page]
11.2.1 Chromatographic conditions
11.2.2 Mobile phase reagents
11.2.3 HPLC separation conditions
11.3 Calculation [Go to Page]
11.3.1 Calibration curve
11.3.2 Sample quantification
12 Repeatability and reproducibility [Go to Page]
12.1 General
12.2 Results from the 2012 collaborative study
13 Test report
Annex A (informative) Recrystallization of 2,4-dinitrophenylhydrazine
Annex B (normative) Example of calibration standards preparation
Annex C (informative) HPLC chromatogram of a typical combined carbonyl calibration standard
Annex D (informative) HPLC chromatogram of carbonyls in DNPH extract of mainstream cigarette smoke
Bibliography [Go to Page]

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BS ISO 21160:2018 Cigarettes. Determination of selected carbonyls in the mainstream smoke of cigarettes. Method using high performance liquid chromatography, 2018

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