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BS ISO 19344:2015 Milk and milk products. Starter cultures, probiotics and fermented products. Quantification of lactic acid bacteria by flow cytometry >
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BS ISO 19344:2015 Milk and milk products. Starter cultures, probiotics and fermented products. Quantification of lactic acid bacteria by flow cytometry, 2015
- Forewords
- Introduction
- 1 Scope
- 2 Normative references
- 3 Terms and definitions
- 4 Principle
- 5 Diluents and reagents
- 5.1 General
- 5.2 Peptone-salt solution
- 5.3 Diluents and reagents for staining protocols
- 5.3.1 Protocol A
- 5.3.2 Protocol B
- 5.3.3 Protocol C
- 6 Apparatus
- 7 Sampling
- 8 Preparation of test sample
- 8.1 General
- 8.2 Freeze-dried cultures
- 8.3 Frozen cultures
- 8.4 Fermented milk products
- 9 Procedure
- 9.1 General
- 9.2 Staining
- 9.2.1 Protocol A
- 9.2.2 Protocol B
- 9.2.3 Protocol C
- 9.3 Flow cytometry analysis
- 9.3.1 General
- 9.3.2 Instruments and settings
- 9.4 Gating
- 9.4.1 General
- 9.4.2 Protocol A
- 9.4.3 Protocol B
- 9.4.4 Protocol C
- 10 Calculation and expression of results
- 11 Critical factors affecting results
- 12 Precision
- 12.1 Interlaboratory test
- 12.2 Repeatability
- 12.3 Reproducibility
- 13 Test report
- Annex A (informative) Diagram of staining protocols
- Annex B (informative) Calculation example of the appropriate sample dilution
- Annex C (informative) Interlaboratory test
- Bibliography [Go to Page]