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BS EN ISO 21528-2:2017 Microbiology of the food chain. Horizontal method for the detection and enumeration of Enterobacteriaceae - Colony-count technique >
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BS EN ISO 21528-2:2017 Microbiology of the food chain. Horizontal method for the detection and enumeration of Enterobacteriaceae - Colony-count technique, 2018
- undefined
- European foreword
- Endorsement notice
- Foreword
- Introduction
- 1 Scope
- 2 Normative references
- 3 Terms and definitions
- 4 Principle
- 4.1 Preparation of initial suspension and decimal dilutions
- 4.2 Isolation and selection for confirmation
- 4.3 Confirmation
- 4.4 Calculation
- 5 Diluent, culture media and reagent
- 6 Equipment and consumables
- 7 Sampling
- 8 Preparation of test sample
- 9 Procedure
- 9.1 General
- 9.2 Test portion, initial suspension and dilutions
- 9.3 Inoculation and incubation
- 9.4 Counting and selection of colonies for confirmation
- 9.5 Subculturing selected colonies
- 9.6 Biochemical confirmation tests
- 9.6.1 Oxidase reaction
- 9.6.2 Fermentation test
- 9.6.3 Interpretation of biochemical tests
- 10 Expression of results
- 11 Performance characteristics of the method
- 11.1 Interlaboratory study
- 11.2 Repeatability limit
- 11.3 Reproducibility limit
- 12 Test report
- 13 Quality assurance
- Annex A (normative) Culture media and reagents
- Annex B (informative) Method validation studies and performance characteristics
- Bibliography [Go to Page]
