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BS EN ISO 18363-2:2018 Animal and vegetable fats and oils. Determination of fatty-acid-bound chloropropanediols (MCPDs) and glycidol by GC/MS - Method using slow alkaline transesterification and measurement for 2-MCPD, 3-MCPD and glycidol, 2018
- National foreword
- European foreword
- Foreword
- Introduction
- 1 Scope
- 2 Normative references
- 3 Terms and definitions
- 4 Principle
- 5 Reagents [Go to Page]
- 5.1 General
- 5.2 Solvents and chemicals [Go to Page]
- 5.2.1 Toluene.
- 5.2.3 Methanol.
- 5.2.5 Ethyl acetate.
- 5.2.6 Diethyl ether.
- 5.2.7 iso-Octane.
- 5.2.8 Sodium sulfate anhydrous, granular.
- 5.3 Standard and reference compounds [Go to Page]
- 5.3.1 2-MCPD.
- 5.3.2 2-MCPD-d5.
- 5.3.3 2-MCPD-1,3-bis-stearoylester *.
- 5.3.4 2-MCPD-d5-1,3-bis-stearoylester *.
- 5.3.5 3-MCPD.
- 5.3.6 3-MCPD-d5.
- 5.3.7 3-MCPD-1,2-bis-palmitoylester *.
- 5.3.8 3-MCPD-d5-1,2-bis-palmitoylester *.
- 5.3.9 Glycidyl oleate*.
- 5.3.10 Glycidyl-d5 oleate*.
- 5.4 Working solutions** [Go to Page]
- 5.4.2 2-MCPD-d5: 10,0 μg/ml in methanol.
- 5.4.3 3-MCPD: 10,0 μg/ml in methanol.
- 5.4.4 3-MCPD-d5: 10,0 μg/ml in methanol.
- 5.4.5 2-MCPD-1,3-bis-stearoyl ester: 29,1 μg/ml in toluene; equivalent to 5,0 μg/ml free 2-MCPD.
- 5.4.6 2-MCPD-d5-1,3-bis-stearoyl ester: 29,3 μg/ml in toluene; equivalent to 5,0 μg/ml free 2-MCPD.
- 5.4.7 3-MCPD-1,2-bis-palmitoyl ester: 26,6 μg/ml in toluene; equivalent to 5,0 μg/ml free 2-MCPD.
- 5.4.8 3-MCPD-d5-1,2-bis-palmitoyl ester: 26,8 μg/ml in toluene; equivalent to 5,0 μg/ml free 2-MCPD.
- 5.4.9 Glycidyl oleate: 22,8 μg/ml in toluene; equivalent to 5,0 μg/ml free glycidol.
- 5.4.10 Glycidyl-d5 oleate: 23,2 μg/ml in toluene; equivalent to 5,0 μg/ml free glycidol.
- 5.5 Other solutions
- 6 Apparatus
- 7 Sample [Go to Page]
- 7.1 Sampling
- 7.2 Preparation of the test sample
- 8 Procedure [Go to Page]
- 8.1 Spiking with surrogate standard and homogenization
- 8.2 Ester cleavage and glycidol transformation
- 8.3 Matrix removal
- 8.4 Derivatization
- 8.5 Gas chromatography/mass spectrometry references
- 9 Expression of results [Go to Page]
- 9.1 Determination of bound glycidol
- 9.2 Determination of bound 2-MCPD
- 9.3 Determination of bound 3-MCPD
- 9.4 Determination of the degree of diester cleavage
- 9.5 Quality control
- 10 Notes
- Annex A (informative) Examples of relevant chromatograms and data evaluation using “low-MCPD” palm oil
- Annex B (informative) Results of interlaboratory tests
- Bibliography [Go to Page]
