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BS EN ISO 16649-3:2015 Microbiology of the food chain. Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli - Detection and most probable number technique using 5-bromo-4-chloro-3-indolyl-β-D-glucuronide >
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BS EN ISO 16649-3:2015 Microbiology of the food chain. Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli - Detection and most probable number technique using 5-bromo-4-chloro-3-indolyl-β-D-glucuronide, 2017
- Contents Page
- Foreword
- Foreword
- Introduction
- 1 Scope
- 2 Normative references
- 3 Terms and definitions
- 4 Principle
- 4.1 Detection method
- 4.2 Enumeration method
- 4.2.1 Inoculation of three or five tubes of double strength liquid selective enrichment medium [5.3.1.1 a)] with an equal volume of the test sample if the initial product is liquid, or with an equal volume of the initial suspension in the case of other pr
- 5 Dilution fluids and culture media
- 5.1 General
- 5.2 Dilution fluids
- 5.3 Culture media
- 5.3.1 Minerals modified glutamate medium (selective enrichment medium)
- 5.3.2 Tryptone bile glucoronide agar (second selective enrichment medium)
- 5.3.3 Performance testing for the quality assurance of the culture media
- 6 Apparatus and glassware
- 7 Sampling
- 8 Preparation of test sample
- 9 Procedure
- 9.1 Detection method
- 9.1.1 Test portion, initial suspension, and dilutions
- 9.1.2 Incubation of selective enrichment medium
- 9.1.3 Subculturing
- 9.1.4 Secondary incubation
- 9.1.5 Examination of the plates
- 9.1.6 Interpretation
- 9.2 Enumeration method
- 9.2.1 Test portion, initial suspension, and dilutions
- 9.2.2 Inoculation of the selective enrichment medium
- 9.2.3 Incubation
- 9.2.4 Subculturing
- 9.2.5 Second incubation
- 9.2.6 Examination of the plates
- 9.2.7 Interpretation
- 10 Expression of results
- 10.1 Detection method
- 10.2 Enumeration method
- 11 Precision
- 12 Test report
- Bibliography
- 64_e_stf EN ISO 16649-3.pdf [Go to Page]
- ISO_16649-3_2015 28E 29-Character_PDF_document.pdf [Go to Page]
