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eLibrary > BS EN ISO 14501:2021 Milk and milk powder. Determination of aflatoxin M1 content. Clean-up by immunoaffinity chromatography and determination by high-performance liquid chromatography >

BS EN ISO 14501:2021 Milk and milk powder. Determination of aflatoxin M1 content. Clean-up by immunoaffinity chromatography and determination by high-performance liquid chromatography, 2021

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BS EN ISO 14501:2021 Milk and milk powder. Determination of aflatoxin M1 content. Clean-up by immunoaffinity chromatography and determination by high-performance liquid chromatography, 2021

National foreword
European foreword
Forewords
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents [Go to Page]
5.1 Immunoaffinity column.
5.3 Nitrogen gas.
5.4 Aflatoxin M1 standard solutions.
6 Apparatus
7 Sampling
8 Procedure [Go to Page]
8.1 General
8.2 Preparation of test samples [Go to Page]
8.2.1 Milk
8.2.2 Milk powder
8.3 Immunoaffinity column preparation
8.4 Test sample purification
8.5 High performance liquid chromatography (HPLC) [Go to Page]
8.5.1 Pump setting
8.5.2 Chromatographic performance
8.5.3 Calibration curve of aflatoxin M1
8.5.4 Analysis of the purified extracts and injection scheme
9 Calculation and expression of results [Go to Page]
9.1 Skimmed milk [Go to Page]
9.1.1 Calculation
9.1.2 Expression of results
9.2 Skimmed milk powder [Go to Page]
9.2.1 Calculation
9.2.2 Expression of results
10 Precision [Go to Page]
10.1 Interlaboratory test
10.2 Repeatability
10.3 Reproducibility
11 Test report
Annex A (informative) Results of an interlaboratory trial
Bibliography [Go to Page]

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BS EN ISO 14501:2021 Milk and milk powder. Determination of aflatoxin M1 content. Clean-up by immunoaffinity chromatography and determination by high-performance liquid chromatography, 2021

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