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eLibrary > 23/30466937 DC BS ISO 24479 Biotechnology — Cellular morphological analysis — General requirements and considerations for cell morphometry to quantify cell morphological features >

23/30466937 DC BS ISO 24479 Biotechnology — Cellular morphological analysis — General requirements and considerations for cell morphometry to quantify cell morphological features, 2023

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23/30466937 DC BS ISO 24479 Biotechnology — Cellular morphological analysis — General requirements and considerations for cell morphometry to quantify cell morphological features, 2023

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ISO_DIS 24479 ed.1 - id.78876 Enquiry PDF (en).pdf [Go to Page]
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Abbreviations
5 General Concept [Go to Page]
5.1 Cell morphology
5.2 Steps for cell morphometry
6 Target of interest (TOI)
7 Image capture [Go to Page]
7.1 Microscopic observation method [Go to Page]
7.1.1 General
7.1.2 Cell properties to be observed
7.1.3 Sample preparation
7.2 Microscope system and its settings [Go to Page]
7.2.1 General
7.2.2 Light source
7.2.3 Objective lens and condenser lens
7.2.4 Components in optical path
7.2.5 Image capture device
7.2.6 Image data
7.2.7 Environmental conditions
7.2.8 Observing position
7.2.9 Image capturing conditions
7.3 Execution of image capture [Go to Page]
7.3.1 Single image capture
7.3.2 Multiple image capture
8 Segmentation of the TOI
9 Quantification [Go to Page]
9.1 General
9.2 Procedure to quantify morphological features of segmented object
10 Qualification of Measurement
11 Reporting [Go to Page]
11.1 General
11.2 Reporting of sample properties and sample preparation
11.3 Reporting of the microscopic observation method
11.4 Reporting of the image data, image pre-processing, and image analysis for morphometric analysis
11.5 Reporting of the morphological features for morphometric analysis
Annex€A (informative) Check sheet regarding selection of contrast-enhancing techniques for optical microscopy
Annex€B (informative) Check sheet regarding selection of microscope system
Annex€C (informative) List of morphological feature parameters, description and formula – Cell shape, size –
Annex€D (informative) List of morphological feature parameters, description and formula – Cell texture –
Annex€E (informative) Points to consider when acquiring phase-contrast images of cells suitable for image analysis
Bibliography [Go to Page]

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23/30466937 DC BS ISO 24479 Biotechnology — Cellular morphological analysis — General requirements and considerations for cell morphometry to quantify cell morphological features, 2023

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