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eLibrary > 23/30451046 DC BS EN ISO 19045-2. Ophthalmic optics. Contact lens care products - Part 2. Method for evaluating disinfecting efficacy by contact lens care products using trophozoites of Acanthamoeba species as the challenge organisms >

23/30451046 DC BS EN ISO 19045-2. Ophthalmic optics. Contact lens care products - Part 2. Method for evaluating disinfecting efficacy by contact lens care products using trophozoites of Acanthamoeba species as the challenge organisms, 2023

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23/30451046 DC BS EN ISO 19045-2. Ophthalmic optics. Contact lens care products - Part 2. Method for evaluating disinfecting efficacy by contact lens care products using trophozoites of Acanthamoeba species as the challenge organisms, 2023

ISO_DIS 19045-2 ed.1 - id.83822 Enquiry PDF (en).pdf [Go to Page]
Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Principle [Go to Page]
4.1 General
5 Acanthamoeba trophozoite disinfecting test method [Go to Page]
5.1 Organisms
5.2 Culture media and reagents
5.3 Test materials
5.4 Test samples
5.5 Culture maintenance
5.6 Growth and harvest of microbial challenge (trophozoite)
5.7 Preparation of Acanthamoeba stock solution
5.8 Stand-alone procedure – inoculation
5.9 Recovery Procedures [Go to Page]
5.9.1 Stand-alone procedure – recovery method one (12 well plate method)
5.9.2 Stand-alone procedure – recovery method two (96 well plate method)
6 Controls [Go to Page]
6.1 Inoculum Control
6.2 Recovery medium control
7 Performance Criteria
Annex€A (normative) Preparation of Acanthamoeba growth medium (Ac#6)
Annex€B (normative) Preparation of ¼ Strength Ringer’s Solution
Annex€C (informative) Maintenance of Acanthamoeba trophozoites and preparation for testing
Annex€D (normative) Preparation of Non-Nutrient Agar (NNA)
Annex€E (informative) Preparation of E.coli Suspension
Annex€F (informative) Preparation of Non-Nutrient Agar (NNA) Plates with E.coli
Annex€G (informative) Preparation of Neutraliser Broth for recovery methods
Annex€H (normative) Reed and Muench Computation Method for Calculation of the 50€% Endpoint Titre
Annex€I (normative) Spearman-Karber Computation Method for Calculation of the 50€% Endpoint Titre
Annex€J (normative) Micrographs of Cultures Using the 96 Well Plate Method
Bibliography [Go to Page]

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23/30451046 DC BS EN ISO 19045-2. Ophthalmic optics. Contact lens care products - Part 2. Method for evaluating disinfecting efficacy by contact lens care products using trophozoites of Acanthamoeba species as the challenge organisms, 2023

⨯To continue with accurate pricing,
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