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eLibrary > 22/30422334 DC BS ISO 24421. Biotechnology. Minimum requirements for optical signal measurements in photometric methods for biological samples >

22/30422334 DC BS ISO 24421. Biotechnology. Minimum requirements for optical signal measurements in photometric methods for biological samples, 2022

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22/30422334 DC BS ISO 24421. Biotechnology. Minimum requirements for optical signal measurements in photometric methods for biological samples, 2022

30422334-NC.pdf
ISO_DIS 24421 ed.1 - id.78742 Enquiry PDF (en).pdf [Go to Page]
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principles [Go to Page]
4.1 General
4.2 Instruments and measurements
4.3 Optical references
5 Minimum requirements to support optical signal measurement [Go to Page]
5.1 Elements of photometric methods
5.2 Verification of optical signal measurement instruments [Go to Page]
5.2.1 Optical references
5.2.2 Dynamic range
5.2.3 Background signals
5.3 Optical signal measurement of biological samples [Go to Page]
5.3.1 Optical signal measurement
5.3.2 Calibration curve
5.3.3 Photometric methods
5.3.4 Personnel
5.4 Data analysis and reporting
Annex€A (informative) Principles of bioluminescence, chemiluminescence, fluorescence and absorption
Annex€B (informative) List of optical references, optical signal measurement instruments, and€representative photometric methods
Annex€C (informative) List of relevant standards describing representative methods by€means of€optical signal measurements
Annex€D (informative) Example of qualification of luminometer using LED reference light source
Annex€E (informative) Example of application of reference light source for comparison measurements of bioluminescent sample using luminometers
Annex€F (informative) Example of determination of well-to-well crosstalk in multi-well plates
Annex€G (informative) Examples of dynamic range determination of luminometer
Annex€H (informative) Example of construction of calibration curve and dynamic range determination of fluorescence plate reader
Annex€I (informative) Example of dynamic range determination of a flow cytometer
Annex€J (informative) Example of calibration of reference light sources and luminometers
Annex€K (informative) Examples of spectral properties of photodetectors
Bibliography [Go to Page]

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22/30422334 DC BS ISO 24421. Biotechnology. Minimum requirements for optical signal measurements in photometric methods for biological samples, 2022

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