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BS EN ISO 18415:2017+A1:2022 Cosmetics. Microbiology. Detection of specified and non-specified microorganisms >
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BS EN ISO 18415:2017+A1:2022 Cosmetics. Microbiology. Detection of specified and non-specified microorganisms, 2022
- undefined [Go to Page]
- Blank Page
- Foreword
- Introduction
- 1 Scope
- 2 Normative references
- 3 Terms and definitions
- 4 Principle
- 5 Diluents and culture media
- 5.1 General
- 5.2 Diluent for the microbial suspension (tryptone sodium chloride solution)
- 5.2.1 General
- 5.2.2 Composition
- 5.2.3 Preparation
- 5.3 Culture media
- 5.3.1 General
- 5.3.2 Enrichment broth
- 5.3.3 Non-selective agar medium
- 6 Apparatus and glassware
- Blank Page
- 7 Strains of microorganism
- 8 Handling of cosmetic products and laboratory samples
- 9 Procedure
- 9.1 General recommendations
- 9.2 Preparation of the initial suspension in the enrichment broth
- 9.2.1 General
- 9.2.2 Water-miscible products
- 9.2.3 Water-immiscible products
- 9.2.4 Filterable products
- 9.3 Incubation of the initial suspension
- 9.4 Isolation of specified and non-specified microorganisms
- 9.5 Procedure for identification of the specified microorganism: Pseudomonas aeruginosa
- 9.5.1 Gram staining
- 9.5.2 Oxidase test
- 9.5.3 Identification test
- 9.6 Procedure for identification of the specified microorganism: Escherichia coli
- 9.6.1 Gram staining
- 9.6.2 Oxidase test
- 9.6.3 Identification test
- 9.7 Procedure for identification of the specified microorganism: Staphylococcus aureus
- 9.7.1 Gram staining
- 9.7.2 Catalase test
- 9.7.3 Identification test
- 9.8 Procedure for the identification of the specified microorganism: Candida albicans
- 9.8.1 Gram staining
- 9.8.2 Identification test
- 9.9 Procedure for the identification of non-specified microorganisms
- 9.9.1 Gram staining
- 9.9.2 Oxidase test
- 9.9.3 Catalase test
- 9.9.4 Identification test
- 10 Expression of the results
- 10.1 Detection of specified microorganisms
- 10.2 Detection of non-specified microorganisms
- 10.3 Absence of microorganisms
- 11 Neutralization of the antimicrobial properties of the product
- 11.1 General
- 11.2 Preparation of inoculum
- 11.3 Suitability of detection method by enrichment
- 11.3.1 Principle
- 11.3.2 Procedure
- 11.3.3 Interpretation of suitability test results
- 12 Test report
- Annex A (informative) General scheme for identification of microorganisms
- Annex B (informative) Other media
- Annex C (informative) Neutralizers of antimicrobial activity of preservatives and rinsing liquids
- Bibliography [Go to Page]