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BS EN ISO 20418-3:2020 Textiles. Qualitative and quantitative proteomic analysis of some animal hair fibres - Peptide detection using LC-MS without protein reduction >
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BS EN ISO 20418-3:2020 Textiles. Qualitative and quantitative proteomic analysis of some animal hair fibres - Peptide detection using LC-MS without protein reduction, 2020
- undefined [Go to Page]
- Foreword
- Introduction
- 1 Scope
- 2 Normative references
- 3 Terms and definitions
- 4 Symbols and abbreviated terms
- 5 Principle
- 6 Reagents
- 7 Apparatus
- 8 Test method
- 8.1 Sampling
- 8.2 Preliminary identification
- 8.3 Wash for degreasing
- 8.4 Powderization of fibres
- 8.5 Trypsin digestion
- 8.6 Marker peptides
- 8.7 LC-MS analysis
- 8.8 Evaluation of the validity of observed data
- 8.9 Calculation of correction factor
- 8.9.1 Correction factor
- 8.9.2 Correction factor for Cas1 against She1 and Yak1 (kc)
- 8.9.3 Correction factor for Yak2 against She2, She3 and Cas2 (ky)
- 8.9.4 Correction factor for alpaca and camel (ka)
- 8.9.5 Correction factor for angora (kr)
- 8.10 Calculation of blending ratio
- 8.10.1 Calculation of blending ratio of cashmere, sheep wool and yak
- 8.10.2 Calculation by She1, Cas1, Yak1 and kc
- 8.10.3 Calculation by She2, She3, Cas2, Yak2 and ky
- 8.10.4 Calculation by She1, She2, She3, Cas2, Yak1
- 8.10.5 Calculation of blending ratio of camel, alpaca and angora
- 9 Test report
- Annex A (informative) Marker peptides of cashmere, sheep wool and yak fibres
- Annex B (informative) Example of LC-MS analysis conditions
- Annex C (informative) Analysis of camel, alpaca and angora fibres
- Annex D (informative) Example of marker peptide mass chromatograms
- Annex E (informative) Results of interlaboratory study
- Bibliography [Go to Page]